Morphology-Preserving Holotomography for 3D Organoid Analysis
Published:Dec 27, 2025 06:07
•1 min read
•ArXiv
Analysis
This paper presents a novel method, Morphology-Preserving Holotomography (MP-HT), to improve the quantitative analysis of 3D organoid dynamics using label-free imaging. The key innovation is a spatial filtering strategy that mitigates the missing-cone artifact, a common problem in holotomography. This allows for more accurate segmentation and quantification of organoid properties like dry-mass density, leading to a better understanding of organoid behavior during processes like expansion, collapse, and fusion. The work addresses a significant limitation in organoid research by providing a more reliable and reproducible method for analyzing their 3D dynamics.
Key Takeaways
- •Introduces Morphology-Preserving Holotomography (MP-HT) to address the missing-cone artifact in holotomography.
- •Provides a 3D segmentation pipeline for robust separation of epithelial and luminal structures.
- •Enables morphology-independent estimation of dry-mass density and total dry mass.
- •Demonstrates the framework's application in analyzing hepatic organoid dynamics during expansion, collapse, and fusion.
Reference
“The results demonstrate consistent segmentation across diverse geometries and reveal coordinated epithelial-lumen remodeling, breakdown of morphometric homeostasis during collapse, and transient biophysical fluctuations during fusion.”