Label-free Brain Organoid Imaging with Fourier Ptychographic Microscopy
Published:Dec 30, 2025 22:17
•1 min read
•ArXiv
Analysis
This paper introduces a novel application of Fourier ptychographic microscopy (FPM) for label-free, high-resolution imaging of human brain organoid slices. It demonstrates the potential of FPM as a cost-effective alternative to fluorescence microscopy, providing quantitative phase imaging and enabling the identification of cell-type-specific biophysical signatures within the organoids. The study's significance lies in its ability to offer a non-invasive and high-throughput method for studying brain organoid development and disease modeling.
Key Takeaways
- •FPM enables label-free, high-resolution imaging of brain organoid slices.
- •FPM provides quantitative phase imaging, revealing cell-type-specific biophysical signatures.
- •The method allows for correlative imaging with fluorescence microscopy.
- •FPM offers a cost-effective and high-throughput approach for studying brain organoid development and disease modeling.
Reference
“Nuclei located in neurogenic regions consistently exhibited significantly higher phase values (optical path difference) compared to nuclei elsewhere, suggesting cell-type-specific biophysical signatures.”