Label-free Brain Organoid Imaging with Fourier Ptychographic Microscopy

This paper introduces a novel application of Fourier ptychographic microscopy (FPM) for label-free, high-resolution imaging of human brain organoid slices. It demonstrates the potential of FPM as a cost-effective alternative to fluorescence microscopy, providing quantitative phase imaging and enabling the identification of cell-type-specific biophysical signatures within the organoids. The study's significance lies in its ability to offer a non-invasive and high-throughput method for studying brain organoid development and disease modeling.